In Vitro Selection of N‐Peptide‐Binding RNA on a Quartz‐Crystal Microbalance to Study a Sequence‐Specific Interaction between the Peptide and Loop RNA
Identifieur interne : 003270 ( Main/Exploration ); précédent : 003269; suivant : 003271In Vitro Selection of N‐Peptide‐Binding RNA on a Quartz‐Crystal Microbalance to Study a Sequence‐Specific Interaction between the Peptide and Loop RNA
Auteurs : Hiroyuki Furusawa ; Akiko Murakawa ; Shinobu Fukusho ; Yoshio Okahata [Japon]Source :
- ChemBioChem [ 1439-4227 ] ; 2003-03-03.
English descriptors
- Teeft :
- Allosteric, Amino, Amino acids, Binding constants, Boxb, Chem, Chembiochem, Effector, External regulation, Furusawa, Gaaga, Gaaga loop, Gcaca, Gcgca, Gcgca loop, Gmbh, Gnrna, Hairpin, Hairpin ribozyme, Hairpin ribozyme activity, Hairpin ribozyme substrate, Hammerhead ribozymes, Hepes, Kgaa, Nacl, Nucleic acids symp, Okahata, Oligonucleotide, Oligonucleotide effector, Pentaloop, Peptide, Random rnas, Randomized, Randomized loop rnas, Ribozyme, Ribozymes, Rna, Saturation binding behavior, Second position, Secondary structure, Selection process, Selection round, Verlag, Verlag gmbh, Weinheim, Weinheim chembiochem.
Abstract
On‐the‐spot RNA selection: N peptide or mutated N peptide was immobilized on a highly sensitive 27‐MHz quartz‐crystal microbalance, which was used as a monitoring and evaluation device for in vitro selection of RNA sequences that bind to these peptides (see figure). This method allowed the study of a sequence‐specific interaction of the peptide with loop RNA in situ and without the use of labeling techniques and time‐consuming separate binding assays.
Url:
DOI: 10.1002/cbic.200390034
Affiliations:
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